Purification and Characterization of a Cytotonic Protein Expressed In Vitro by the Live Cholera Vaccine Candidate CVD 103-HgR

@article{Sathyamoorthy2000PurificationAC,
  title={Purification and Characterization of a Cytotonic Protein Expressed In Vitro by the Live Cholera Vaccine Candidate CVD 103-HgR},
  author={V. Sathyamoorthy and R. H. Hall and B. Mccardell and M. Kothary and S. Ahn and S. Ratnayake},
  journal={Infection and Immunity},
  year={2000},
  volume={68},
  pages={6062 - 6065}
}
  • V. Sathyamoorthy, R. H. Hall, +3 authors S. Ratnayake
  • Published 2000
  • Biology, Medicine
  • Infection and Immunity
  • ABSTRACT Cholera vaccines developed by the deletion of CTX genes fromVibrio cholerae induce a residual reactogenicity in up to 10% of vaccinees. A novel cytotonic agent named secreted CHO cell elongating protein (S-CEP) was purified from culture supernatants of CVD 103-HgR (Levine et al., Lancet ii:467–470, 1988). Five fractionation steps yielded electrophoretically pure S-CEP with anMr of 79,000. A partially purified preparation caused fluid accumulation in the sealed infant mouse model. The… CONTINUE READING
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