Purification and Characterization of a Membrane-Associated 48-Kilodalton Phospholipase A 2 in Leaves of Broad Bean 1

Abstract

Several lines of evidence indicate that phospholipase A2 (PLA2) plays a crucial role in plant cellular responses through production of linolenic acid, the precursor of jasmonic acid, from membrane phospholipids. Here we report the purification and characterization of a 48-kD PLA2 from the membrane fractions of leaves of broad bean (Vicia faba). The plant… (More)

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