Purification and Characterization of a 43-kDa Rotenone-insensitive NADH Dehydrogenase from Plant Mitochondria*

@article{Menz1996PurificationAC,
  title={Purification and Characterization of a 43-kDa Rotenone-insensitive NADH Dehydrogenase from Plant Mitochondria*},
  author={R. Menz and D. Day},
  journal={The Journal of Biological Chemistry},
  year={1996},
  volume={271},
  pages={23117 - 23120}
}
  • R. Menz, D. Day
  • Published 1996
  • Biology, Medicine
  • The Journal of Biological Chemistry
A 43-kDa NAD(P)H dehydrogenase was purified from red beetroot mitochondria. An antibody against this dehydrogenase was used in conjunction with the membrane-impermeable protein cross-linker 3,3′-dithiobis(sulfosuccinimidylpropionate) to localize the dehydrogenase on the matrix side of the inner membrane. Immunoblotting showed that the dehydrogenase was found in mitochondria isolated from several plant species but not from rat livers. Antibodies against the purified dehydrogenase partially… Expand
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  • Biology, Medicine
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TLDR
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