Purification and Characterization of Two Invertases from Mutant Strain of Saccharomyces Cerevisiae

@inproceedings{Aslam2013PurificationAC,
  title={Purification and Characterization of Two Invertases from Mutant Strain of Saccharomyces Cerevisiae},
  author={Aafia Aslam and Ikram-ul-Haq},
  year={2013}
}
In the present study, a mutant strain of Saccharomyces cerevisae EMS-42 was used for the biosynthesis of invertase (E.C.3.2.1.26). Both types of invertases i.e., extracellular and intracellular invertase are present in S. cerevisae. An extracellular invertase was purified to homogeneity by two step purification i.e., ammonium sulfate precipitation and DEAE-Sephadex A-50. The enzyme was present in the supernatant of 85% saturation being glycoprotein in nature. DEAE column chromatography eluted… CONTINUE READING

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