• Corpus ID: 33469982

Purification and Characterization of Tannin Acyl Hydrolase from Aspergillus niger ATCC 16620

@article{Sabu2005PurificationAC,
  title={Purification and Characterization of Tannin Acyl Hydrolase from Aspergillus niger ATCC 16620},
  author={A. Sabu and Gopikrishnan Kiran and Ashok Pandey},
  journal={Food Technology and Biotechnology},
  year={2005},
  volume={43},
  pages={133-138}
}
Summary Tannin acyl hydrolase produced extracellularly by the fungal strain Aspergillus niger ATTC 16620 in solid state fermentation was purified from the cell free culture broth by ammonium sulphate fractionation followed by DEAE–Sephadex A-50 chromatography. SDS-PAGE analysis indicated that the enzyme protein molecular mass was 168 kDa. Enzyme activity was stable up to the temperature of 40 °C and the enzyme activity was optimal at pH=6. Tannase activity was maximal at 0.01 M concentration of… 
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