Purification and Characterization of Polyphenol Oxidase from Glandular Trichomes of Solanum berthaultii.

  title={Purification and Characterization of Polyphenol Oxidase from Glandular Trichomes of Solanum berthaultii.},
  author={Susanne Kowalski and Nancy T. Eannetta and A T Hirzel and John C. Steffens},
  journal={Plant physiology},
  volume={100 2},
Type A glandular trichomes of the wild potato (Solanum berthaultii Hawkes) entrap insects by rapidly polymerizing the trichome contents after breakage by insect contact. Polymerization of trichome exudate appears to be driven by a soluble polyphenol oxidase (PPO). PPO constitutes up to 70% of the protein in individually collected trichomes and reaches a concentration approaching 200 mum in these organs. Trichome PPO has been purified and shown to be a monomeric copper metalloprotein with an… CONTINUE READING


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