Purification and Characterization of Phosphoenolpyruvate Carboxylase from Developing Seeds of Brassica

Abstract

Phosphoenolpyruvate (PEP) carboxylase (EC 4.1.1.31) was purified to apparent homogeneity with about 29% recovery from developing seeds of Brassica using ammonium sulfate fractionation, DEAE-cellulose chromatography, and gel filtration through Sepharose CL-6S. The purified enzyme with mol wt of about 400 kD exhibited maximum activity at pH 8.0. The enzyme had an absolute requirement for a divalent cation which was satisfied by Mg2+. The enzyme showed typical hyperbolic kinetics with PEP and HCO−3 with Km of 0.125 and 0.104 mM, respectively. Glu-6-P could activate the enzyme, whereas other phosphate esters such as fru-1, 6-P2, L-glycerophosphate and 3-PGA did not have any effect on the enzyme activity. Noneof the amino acids at 5 mM concentration had any significant effect on the enzyme activity. Nucleotide monophosphates and diphosphates did not inhibit the enzyme significantly, whereas ATP inhibited the enzyme activity. Oxaloacetate and malate inhibited the enzyme non-competitively with respect to PEP with Ki values of 0.127 and 1.25 mM, respectively. The enzyme activity in vivo seems to be regulated ’Tlainly by availability of its substrate and activation by glu-6-P, both of which are supplied through glycolysis.

DOI: 10.1007/BF03262943

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Cite this paper

@article{Mehta2012PurificationAC, title={Purification and Characterization of Phosphoenolpyruvate Carboxylase from Developing Seeds of Brassica}, author={Maneesh Mehta and M. R. Saharan and Randhir Singh}, journal={Journal of Plant Biochemistry and Biotechnology}, year={2012}, volume={4}, pages={11-16} }