Purification and Characterization of Mouse Kidney , & Glucuronidase

  title={Purification and Characterization of Mouse Kidney , & Glucuronidase},
  author={C Lin and M. Orcutt and William H. Fishman},
P-Glucuronidase has been purified from mouse kidneys previously induced by gonadotrophin to a specific enzyme activity 15 times higher than the non-induced kidney. The purification procedure includes ultrasonication to solubilize the enzyme, acid and ammonium sulfate precipitations, gel filtration in Sephadex G-200, DEAE-ion exchange chromatography, and isoelectric focusing. The resulting product has a specific activity of 284,000 Fishman units/mg of protein, representing a 1,090.fold… CONTINUE READING