Purification and Characterization of Milk Clotting Enzyme Produced by Bacillus sphaericus

@inproceedings{ElBendary2007PurificationAC,
  title={Purification and Characterization of Milk Clotting Enzyme Produced by Bacillus sphaericus},
  author={Magda A. El-Bendary and Maysa E. Moharam and Thanaa H. Ali},
  year={2007}
}
Milk cl otting e nzyme (MCE) prod uced by Egyptian Bacillus sphaericus NRC 24 was p artially purified and characterized. M CE was ob tained by fractional precipitation wi th acet one, followed by the chromatography of the most active fraction on DEAE-Sephadex A-25 and finally on Sephadex G-100 with 48 purification f old and specific a ctivity a bout 648148 U /mg prot ein. The maximum enzyme act ivity was at a w ide range of pHs (5.7-7.5) a nd 55oC. The clotting activity of the purified en zyme… CONTINUE READING

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