Purification and Characterization of Leech Hyaluronic Acid-endo- P-glucuronidase*


In 1960, Linker, Meyer, and Hoffman (1) reported that a crude leech preparation hydrolyzes the glucuronide linkages of hyaluronic acid, unlike bacterial and testes hyaluronidases, which act on glucosaminide linkages. This paper deals with the first systematic attempt to purify the leech P-glucuronidase that specifically hydrolyzes hyaluronic acid. Purified… (More)


4 Figures and Tables