Purification and Characterization of Extracellular Pectinesterases from Phytophthora infestans.

@article{Frster1985PurificationAC,
  title={Purification and Characterization of Extracellular Pectinesterases from Phytophthora infestans.},
  author={Hans-Peter F{\"o}rster and Ihab Rasched},
  journal={Plant physiology},
  year={1985},
  volume={77 1},
  pages={109-12}
}
Constitutively produced extracellular pectinesterases from culture filtrates of the potato late blight fungus Phytophthora infestans were purified and characterized. One enzyme (PE II) was purified to homogeneity. Sodium dodecyl sulfate electrophoresis of the second enzyme (PE I) revealed two protein bands; there are indications that both proteins are pectinesterases, which were not separable by a number of different techniques. Thus, P. infestans might produce three pectinesterases in vitro… CONTINUE READING
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