Purification and Characterization of Cloned

  title={Purification and Characterization of Cloned},
  author={Jack and {\'E}. and Baldwin and Stephen and J.. and Killin and Andrew and Pratt and C John and David S Sutherland and P Nicholas and Turner and Michael C James and Charlene Crabbe and Prince Edward and Abraham and Anthony and C. WiLLisn},
  • Jack, É., +15 authors C. WiLLisn
  • Published 2006
Isopenicillin N synthetase (IPS) cloned from Cephalosporium acremonium has been isolated from transformed Escherichia coli and purified to homogeneity. The resulting, abundant, recombinant protein, whilst undergoing slightly different JV-terminal processing to that observed for the fungally-derived protein, has identical kinetics for the conversion of LLD-aminoadipoyl-cysteinyl-valine to isopenicillin N. Recombinant IPS converts analogue substrates into unusual /3-lactam antibiotics in exactly… CONTINUE READING