Purification and Characterization of Allene Oxide Cyclase from Dry Corn Seeds.

@article{Ziegler1997PurificationAC,
  title={Purification and Characterization of Allene Oxide Cyclase from Dry Corn Seeds.},
  author={Joerg Ziegler and Mats H{\'a}mberg and Otto Miersch and Benno Parthier},
  journal={Plant physiology},
  year={1997},
  volume={114 2},
  pages={565-573}
}
Allene oxide cyclase (AOC; EC 5.3.99.6) catalyzes the cyclization of 12,13(S)-epoxy-9(Z),11,15(Z)-octadecatrienoic acid to 12-oxo- 10,15(Z)-phytodienoic acid, the precursor of jasmonic acid (JA). This soluble enzyme was purified 2000-fold from dry corn (Zea mays L.) kernels to apparent homogeneity. The dimeric protein has a molecular mass of 47 kD. Allene… CONTINUE READING