Corpus ID: 42740734

Purification and Characterization of 6-Chlorohydroxyquinol 1 , 2-Dioxygenase from Streptomyces rochei 303 : Comparison with an Analogous Enzyme from Azotobacter sp . Strain GP 1

  title={Purification and Characterization of 6-Chlorohydroxyquinol 1 , 2-Dioxygenase from Streptomyces rochei 303 : Comparison with an Analogous Enzyme from Azotobacter sp . Strain GP 1},
  author={Olga E. Zaborina and Mark Latus and J{\"u}rgen Ebersp{\"a}cher and Ludmila A. Golovleva and Franz Lingens},
The enzyme which cleaves the benzene ring of 6-chlorohydroxyquinol was purified to apparent homogeneity from an extract of 2,4,6-trichlorophenol-grown cells of Streptomyces rochei 303. Like the analogous enzyme from Azotobacter sp. strain GP1, it exhibited a highly restricted substrate specificity and was able to cleave only 6-chlorohydroxyquinol and hydroxyquinol and not catechol, chlorinated catechols, or pyrogallol. No extradiolcleaving activity was observed. In contrast to 6… Expand

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