Purification, thioredoxin renaturation, and reconstituted activity of the three subunits of the influenza A virus RNA polymerase.

Abstract

The virion-associated RNA polymerase and the structural nucleoprotein of influenza A virus were separated by sodium dodecyl sulfate/PAGE, electroblotted to a polyvinylidine membrane, and eluted with good recovery from the membrane. After renaturation by incubating with Escherichia coli thioredoxin, these proteins were active in a reconstituted in vitro… (More)

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@article{Szewczyk1988PurificationTR, title={Purification, thioredoxin renaturation, and reconstituted activity of the three subunits of the influenza A virus RNA polymerase.}, author={Bernadeta Szewczyk and William Graeme Laver and Debbie Summers}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={1988}, volume={85 21}, pages={7907-11} }