Purification, microsequencing, and immunolocalization of p36, a new interferon-alpha-induced protein that is associated with human lupus inclusions.

@article{Rich1996PurificationMA,
  title={Purification, microsequencing, and immunolocalization of p36, a new interferon-alpha-induced protein that is associated with human lupus inclusions.},
  author={Steven A. Rich and Mohua Bose and Paul Tempst and Ulrich H. Rudofsky},
  journal={The Journal of biological chemistry},
  year={1996},
  volume={271 2},
  pages={1118-26}
}
The trace interferon-alpha-induced protein, p36, was induced in Raji cells in association with lupus inclusions. It was solubilized in a nonionic detergent buffer, enriched by differential centrifugation and by preparative isoelectric focusing, and purified to homogeneity on two-dimensional protein gels. Failure to obtain N-terminal amino acid sequence, however, suggested a blocked alpha-amino group. Sequences of six tryptic peptides, 13-19 amino acids in length, were obtained after digestion… CONTINUE READING