Purification, kinetical and molecular characterizations of a serine collagenolytic protease from greenshore crag (Carcinus maenas) digestive gland.

@article{Roy1996PurificationKA,
  title={Purification, kinetical and molecular characterizations of a serine collagenolytic protease from greenshore crag (Carcinus maenas) digestive gland.},
  author={Polly Roy and Bernard Colas and Philippe Durand},
  journal={Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology},
  year={1996},
  volume={115 1},
  pages={87-95}
}
A serine collagenolytic protease was purified from a water soluble fraction of greenshore crab digestive gland by acidic precipitation, gel filtration on a Sephadex G-50 column, ion-exchange chromatography on a Fractogel TSK DEAE column, immobilized metal ion affinity chromatography (IMAC) on IDA (Cu2+) Sepharose 6B and ion-exchange chromatography on Hyper… CONTINUE READING