Purification, identification, and functional analysis of polysomes from the human pathogen Staphylococcus aureus.


Polysomes are macromolecular complexes made up of multiple ribosomes simultaneously translating a single mRNA into polypeptide chains. Together, the cellular mRNAs translated in this way are referred to 'translatome.' Translation determines a cell's overall gene expression profile. Studying translatome leads to a better understanding of the translational… (More)
DOI: 10.1016/j.ymeth.2016.10.003

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