Purification, crystallization and preliminary crystallographic analysis of DehI, a group I alpha-haloacid dehalogenase from Pseudomonas putida strain PP3.

@article{Schmidberger2008PurificationCA,
  title={Purification, crystallization and preliminary crystallographic analysis of DehI, a group I alpha-haloacid dehalogenase from Pseudomonas putida strain PP3.},
  author={Jason W. Schmidberger and Jacqueline A Wilce and Andrew Weightman and Matthew C. J. Wilce},
  journal={Acta crystallographica. Section F, Structural biology and crystallization communications},
  year={2008},
  volume={64 Pt 7},
  pages={596-8}
}
Pseudomonas putida strain PP3 produces two dehalogenases, DehI and DehII, which belong to the group I and II alpha-haloacid dehalogenases, respectively. Group I dehalogenases catalyse the removal of halides from D-haloalkanoic acids and in some cases also the L-enantiomers, both substituted at their chiral centres. Studies of members of this group have resulted in the proposal of general catalytic mechanisms, although no structural information is available in order to better characterize their… CONTINUE READING