Purification, crystallization and preliminary X-ray diffraction analysis of the yeast phosphorelay protein YPD1.

@article{Xu1999PurificationCA,
  title={Purification, crystallization and preliminary X-ray diffraction analysis of the yeast phosphorelay protein YPD1.},
  author={Qingping Xu and Van Dy Nguyen and Ann H. West},
  journal={Acta crystallographica. Section D, Biological crystallography},
  year={1999},
  volume={55 Pt 1},
  pages={291-3}
}
YPD1 is a yeast osmoregulatory protein that functions in a phosphorelay signal-transduction pathway. YPD1 has been expressed in Escherichia coli, purified to homogeneity and crystallized. The crystals were obtained by hanging-drop vapor-diffusion using PEG 4000 as a precipitant. Preliminary X-ray diffraction analysis indicates that the crystals belong to… CONTINUE READING