Purification, cloning and autoproteolytic processing of an aspartic proteinase from Centaurea calcitrapa.

Abstract

Plant aspartic proteinases (APs) have been isolated from several seed and leaf sources but the only well characterized enzymes from flowers are cardosins and cyprosins from cardoon, Cynara cardunculus L. Here we report a full-length cDNA clone encoding an AP named cenprosin from the flowers of Centaurea calcitrapa L., a thistle related to cardoon. As found… (More)

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Cite this paper

@article{Domingos2000PurificationCA, title={Purification, cloning and autoproteolytic processing of an aspartic proteinase from Centaurea calcitrapa.}, author={Ana Domingos and Paula Christina Figueira Cardoso and Zehnyi Xue and Alberto Clemente and Peter E. Brodelius and M. S. Pais}, journal={European journal of biochemistry}, year={2000}, volume={267 23}, pages={6824-31} }