Purification, cloning, and DNA sequence analysis of a chitinase from an overproducing mutant of Streptomyces peucetius defective in daunorubicin biosynthesis.

Abstract

Extracellular chitinases of Streptomyces peucetius and a chitinase overproducing mutant, SPVI, were purified to homogeneity by ion exchange and gel filtration chromatography. The purified enzyme has a molecular mass of 42 kDa on SDS-PAGE, and the N-terminal amino acid sequence of the protein from the wild type showed homology to catalytic domains (Domain IV… (More)

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@article{Vetrivel2001PurificationCA, title={Purification, cloning, and DNA sequence analysis of a chitinase from an overproducing mutant of Streptomyces peucetius defective in daunorubicin biosynthesis.}, author={Kulandaivelu S. Vetrivel and Shunmugiah Karutha Pandian and Ujwal Chaudhary and Komalavali Dharmalingam}, journal={Canadian journal of microbiology}, year={2001}, volume={47 3}, pages={179-87} }