Purification, characterization and substrate specificities of xylanase isoenzymes from Melanocarpus albomyces IIS 68.

The ascomycetous fungus Melanocarpus albomyces when grown on wheat straw produced seven extracellular xylanase isoenzymes, designated as la, Ib, Ic, IIa, lib, llc, and lId. All seven xylanases were purified to homogeneity by gel filtration and ion-exchange chromatography. The molecular mass (kDa) of la, lb, Ic, Ila, lIb, lIc, and lId were estimated to be 22… CONTINUE READING