Purification, characterization and expression of two vitellogenins in the Indian freshwater murrel Channa punctatus.

Abstract

The present study was undertaken to characterize different vitellogenins in Channa punctatus. Protein purification by gel chromatography followed by fast protein liquid chromatography (FPLC) revealed existence of two different Vg forms. Liquid chromatography tandem mass spectrophotometry (LC-MS/MS) suggested the existence of Vga and Vgb. Cloning of partial… (More)
DOI: 10.1016/j.ygcen.2013.05.002

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