Purification, characterization and crystallization of the core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum.

@article{Suzuki2007PurificationCA,
  title={Purification, characterization and crystallization of the core complex from thermophilic purple sulfur bacterium Thermochromatium tepidum.},
  author={Hiroaki Suzuki and Yu Hirano and Yukihiro Kimura and Shinichi Takaichi and Masayuki Kobayashi and Kunio Miki and Zheng-Yu Wang},
  journal={Biochimica et biophysica acta},
  year={2007},
  volume={1767 8},
  pages={1057-63}
}
A light-harvesting-reaction center (LH1-RC) core complex has been highly purified from a thermophilic purple sulfur bacterium, Thermochromatium tepidum. The bacteriochlorophyll (BChl) a molecules in the LH1 exhibit a Q(y) transition at 914 nm, more than 25 nm red-shift from those of its mesophilic counterparts. The LH1-RC complex was isolated in a monomeric form as confirmed by sucrose density gradient centrifugation, blue native PAGE and size-exclusion chromatography. Four subunits (L, M, H… CONTINUE READING

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