Purification, characterization, and crystallization of the components of the nitrobenzene and 2-nitrotoluene dioxygenase enzyme systems.

@article{Parales2005PurificationCA,
  title={Purification, characterization, and crystallization of the components of the nitrobenzene and 2-nitrotoluene dioxygenase enzyme systems.},
  author={Rebecca E. Parales and Rick Huang and C N Yu and Juanito V. Parales and Fook Kay Lee and Daniel Lessner and Maja M Ivkovic-Jensen and Weidong Liu and Rosmarie Friemann and Subramanyan Ramaswamy and D T Gibson},
  journal={Applied and environmental microbiology},
  year={2005},
  volume={71 7},
  pages={3806-14}
}
The protein components of the 2-nitrotoluene (2NT) and nitrobenzene dioxygenase enzyme systems from Acidovorax sp. strain JS42 and Comamonas sp. strain JS765, respectively, were purified and characterized. These enzymes catalyze the initial step in the degradation of 2-nitrotoluene and nitrobenzene. The identical shared reductase and ferredoxin components were monomers of 35 and 11.5 kDa, respectively. The reductase component contained 1.86 g-atoms iron, 2.01 g-atoms sulfur, and one molecule of… CONTINUE READING

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