Purification, cDNA cloning and modification of a defensin from the coconut rhinoceros beetle, Oryctes rhinoceros.

@article{Ishibashi1999PurificationCC,
  title={Purification, cDNA cloning and modification of a defensin from the coconut rhinoceros beetle, Oryctes rhinoceros.},
  author={Jun Ishibashi and Hisako Saido-Sakanaka and J Yang and Aki Sagisaka and Minoru Yamakawa},
  journal={European journal of biochemistry},
  year={1999},
  volume={266 2},
  pages={616-23}
}
A novel member of the insect defensins, a family of antibacterial peptides, was purified from larvae of the coconut rhinoceros beetle, Oryctes rhinoceros, immunized with Escherichia coli. A full-size cDNA was cloned by combining reverse-transcription PCR (RT-PCR), and 5'- and 3'-rapid amplification of cDNA ends (RACE). Analysis of the O. rhinoceros defensin gene expression showed it to be expressed in the fat body and hemocyte, midgut and Malpighian tubules. O. rhinoceros defensin showed strong… CONTINUE READING

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