Purification, cDNA cloning, and developmental changes in the steady-state mRNA level of rat testicular tissue inhibitor of metalloproteases-2 (TIMP-2).

@article{Grima1996PurificationCC,
  title={Purification, cDNA cloning, and developmental changes in the steady-state mRNA level of rat testicular tissue inhibitor of metalloproteases-2 (TIMP-2).},
  author={Josephine Grima and K Calcagno and C. Yan Cheng},
  journal={Journal of andrology},
  year={1996},
  volume={17 3},
  pages={263-75}
}
Using multiple high-performance liquid chromatography (HPLC) steps and high-performance electrophoresis chromatography (HPEC) in conjunction with an [125I]collagen film assay to identify inhibitors of metalloproteases, we have purified a 22-kDa polypeptide to apparent homogeneity from primary Sertoli cell-enriched culture medium. Partial N-terminal amino acid sequence analysis revealed that this protein is similar to the human tissue inhibitor of metalloproteases-2 (TIMP-2). To determine the… CONTINUE READING
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Pure Sertoli cells cultures : a new method for the study of somatic - germ cell interactions

  • M Galdieri, E Ziparo, F Palombi, MA Russo, M Stefanini
  • J Androl
  • 1981
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