Purification, biochemical characterization and protein-DNA interactions of the I-CreI endonuclease produced in Escherichia coli.


I- CreI is a member of the LAGLI-DADG family of homing nucleases; however, unlike most members of this family it contains only a single copy of this signature motif. I- CreI was over-expressed in Escherichia coli, and a simple purification protocol developed that gave reasonably pure protein in high yield. Size-exclusion chromatography and chemical cross… (More)


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