Purification, Partial Characterization and Immobilization of a Mannose-Specific Lectin from Seeds of Dioclea lasiophylla Mart

@inproceedings{Jnior2013PurificationPC,
  title={Purification, Partial Characterization and Immobilization of a Mannose-Specific Lectin from Seeds of Dioclea lasiophylla Mart},
  author={Vanir Reis Pinto J{\'u}nior and Mayara Queiroz de Santiago and Vinicius Jos{\'e} Silva Osterne and Jorge Lu{\'i}s Almeida Correia and Francisco Nascimento Pereira J{\'u}nior and Jo{\~a}o Batista Cajazeiras and Mayron Alves de Vasconcelos and Edson H Teixeira and Ant{\^o}nia S{\^a}mia Fernandes do Nascimento and Thaiz Batista Azevedo Rangel Miguel and Emilio de Castro Miguel and Alexandre Holanda Sampaio and Kyria Santiago do Nascimento and Celso S Nagano and Benildo Sousa Cavada},
  booktitle={Molecules},
  year={2013}
}
Lectin from the seeds of Dioclea lasiophylla (DlyL) was purified in a single step by affinity chromatography on a Sephadex® G-50 column. DlyL strongly agglutinated rabbit erythrocytes and was inhibited by monosaccharides (D-mannose and α-methyl-D-mannoside) and glycoproteins (ovalbumin and fetuin). Similar to other Diocleinae lectins, DlyL has three chains, α, β and γ, with mass of 25,569 ± 2, 12,998 ± 1 and 12,588 ± 1 Da, respectively, and has no disulfide bonds. The hemagglutinating activity… CONTINUE READING

Similar Papers

Citations

Publications citing this paper.
SHOWING 1-10 OF 10 CITATIONS

References

Publications referenced by this paper.
SHOWING 1-10 OF 36 REFERENCES

Isolation and partial characterization of a lectin from Dioclea rostrata

B. S. Cavada, T. B. Grangeiro, +3 authors R. A. Moreira
  • Benth seeds. Rev. Bras. Fisiol. Veg. 1996,
  • 2013
VIEW 1 EXCERPT

Purification and partial characterization of a novel lectin from Dioclea lasiocarpa Mart seeds with vasodilator effects

A.S.F. Nascimento, A.C.S. Gondim, +7 authors B. S. Cavada
  • J. Mol. Recognit
  • 2012
VIEW 1 EXCERPT

Molecular characterization and tandem mass spectrometry of the lectin extracted from the seeds of Dioclea sclerocarpa Ducke

J.L.A. Correia, A.S.F. Nascimento, +7 authors K. S Nascimento
  • Molecules 2011,
  • 2011
VIEW 1 EXCERPT