Purification, Characterization, and Gene Cloning of Purine Nucleosidase from Ochrobactrum anthropi

@article{Ogawa2001PurificationCA,
  title={Purification, Characterization, and Gene Cloning of Purine Nucleosidase from Ochrobactrum anthropi},
  author={J. Ogawa and S. Takeda and S. Xie and H. Hatanaka and T. Ashikari and T. Amachi and S. Shimizu},
  journal={Applied and Environmental Microbiology},
  year={2001},
  volume={67},
  pages={1783 - 1787}
}
ABSTRACT A bacterium, Ochrobactrum anthropi, produced a large amount of a nucleosidase when cultivated with purine nucleosides. The nucleosidase was purified to homogeneity. The enzyme has a molecular weight of about 170,000 and consists of four identical subunits. It specifically catalyzes the irreversibleN-riboside hydrolysis of purine nucleosides, theKm values being 11.8 to 56.3 μM. The optimal activity temperature and pH were 50°C and pH 4.5 to 6.5, respectively. Pyrimidine nucleosides… Expand
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