Purification, Characterization, and Antifungal Activity of Chitinase from Streptomyces venezuelae P10

@article{Mukherjee2005PurificationCA,
  title={Purification, Characterization, and Antifungal Activity of Chitinase from Streptomyces venezuelae P10},
  author={Gurunath Mukherjee and Sukanta Kumar Sen},
  journal={Current Microbiology},
  year={2005},
  volume={53},
  pages={265-269}
}
Streptomyces venezuelae P10 could produce extracellular chitinase in a medium containing 0.6% colloidal chitin that was fermented for 96 hours at 30°C. The enzyme was purified to apparent homogeneity with 80% saturation of ammonium sulfate as shown by chitin affinity chromatography and DEAE-cellulose anion-exchange chromatography. Sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) of the enzyme showed a molecular weight of 66 kDa. The chitinase was characterized, and… CONTINUE READING
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