Puri ® cation and characterisation of a novel DNA methyltransferase ,


We have cloned the M and S genes of the restrictionmodi®cation (R-M) system AhdI and have puri®ed the resulting methyltransferase to homogeneity. M.AhdI is found to form a 170 kDa tetrameric enzyme having a subunit stoichiometry M2S2 (where the M and S subunits are responsible for methylation and DNA sequence speci®city, respectively). Sedimentation… (More)

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