Pulsed interleaved excitation fluctuation imaging.

  title={Pulsed interleaved excitation fluctuation imaging.},
  author={Jelle Hendrix and Waldemar Schrimpf and Matthias H{\"o}ller and Don C Lamb},
  journal={Biophysical journal},
  volume={105 4},
Fluorescence fluctuation imaging is a powerful means to investigate dynamics, interactions, and stoichiometry of proteins inside living cells. Pulsed interleaved excitation (PIE) is the method of nanosecond alternating excitation with time-resolved detection and allows accurate, independent, and quasi-simultaneous determination of fluorescence intensities and lifetimes of different fluorophores. In this work, we combine pulsed interleaved excitation with fluctuation imaging methods (PIE-FI… CONTINUE READING
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