Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes

@article{Pyle1988PulsedfieldEI,
  title={Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes},
  author={L. E. Pyle and L. N. Corcoran and B. G. Cocks and A. D. Bergemann and J. C. Whitley and L. R. Finch},
  journal={Nucleic acids research},
  year={1988},
  volume={16 13},
  pages={6015-25}
}
The sizes of large DNA fragments produced from genomes of members of the Mycoplasmataceae by digestion with restriction endonucleases having infrequent (1 to 3) cleavage sites within the genome were estimated from their mobility in contour-clamped homogeneous electric field (CHEF) agarose gel electrophoresis by comparison with yeast chromosomal DNA markers. The estimates of total genome size for 7 strains of 6 species ranged from approximately 900 kilo base pairs (kb) for Ureaplasma urealyticum… CONTINUE READING