Pulse proteolysis: A simple method for quantitative determination of protein stability and ligand binding
@article{Park2005PulsePA, title={Pulse proteolysis: A simple method for quantitative determination of protein stability and ligand binding}, author={C. Park and S. Marqusee}, journal={Nature Methods}, year={2005}, volume={2}, pages={207-212} }
Thermodynamic stability is fundamental to the biology of proteins. Information on protein stability is essential for studying protein structure and folding and can also be used indirectly to monitor protein-ligand or protein-protein interactions. While clearly valuable, the experimental determination of a protein's stability typically requires biophysical instrumentation and substantial quantities of purified protein, which has limited the use of this technique as a general laboratory method… CONTINUE READING
208 Citations
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