Pulse proteolysis: A simple method for quantitative determination of protein stability and ligand binding

  title={Pulse proteolysis: A simple method for quantitative determination of protein stability and ligand binding},
  author={C. Park and S. Marqusee},
  journal={Nature Methods},
  • C. Park, S. Marqusee
  • Published 2005
  • Medicine
  • Nature Methods
  • Thermodynamic stability is fundamental to the biology of proteins. Information on protein stability is essential for studying protein structure and folding and can also be used indirectly to monitor protein-ligand or protein-protein interactions. While clearly valuable, the experimental determination of a protein's stability typically requires biophysical instrumentation and substantial quantities of purified protein, which has limited the use of this technique as a general laboratory method… CONTINUE READING
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