• DOI:10.1038/nmeth740
Corpus ID: 21364478

Pulse proteolysis: A simple method for quantitative determination of protein stability and ligand binding

@article{Park2005PulsePA,
  title={Pulse proteolysis: A simple method for quantitative determination of protein stability and ligand binding},
  author={C. Park and S. Marqusee},
  journal={Nature Methods},
  year={2005},
  volume={2},
  pages={207-212}
}

C. Park, S. Marqusee

Published 2005

Medicine

Nature Methods

Thermodynamic stability is fundamental to the biology of proteins. Information on protein stability is essential for studying protein structure and folding and can also be used indirectly to monitor protein-ligand or protein-protein interactions. While clearly valuable, the experimental determination of a protein's stability typically requires biophysical instrumentation and substantial quantities of purified protein, which has limited the use of this technique as a general laboratory method… CONTINUE READING

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