Pulse Labelled RNA associated with Ribosomes in Escherichia coli

Pulse-labelling studies involving sucrose gradient centrifugation in changing concentrations of magnesium ions have been used to demonstrate that newly synthesized RNA, contrary to the current view, is not reversibly bound to 70S ribosomes but is attached even in low concentrations of magnesium ions to both 5OS and 30S ribosomal sub-units