PURPOSE To determine the normal reference range of pulsatile ocular blood flow (POBF) values in healthy Korean subjects and to find out the factors that may affect them. METHODS A total of 280 eyes of 280 normal subjects were included in this study. Best corrected visual acuity (BCVA), intraocular pressure (IOP), axial length, POBF, systemic blood pressure, and pulse rate were measured. The mean, standard deviation, range, and the 5th and 95th percentiles of POBF were calculated, and the influences of various parameters to POBF were determined by multiple regression analyses. RESULTS The mean POBF value was 766.0+/-221.6 microl/min in men and 1021.1+/-249.5 microl/min in women. The 5th and 95th percentiles for POBF values were 486.0 microl/min and 1140.0 microl/min in men and 672.0 microl/min and 1458.0 microl/min in women. The POBF values were significantly influenced by gender, mean blood pressure, pulse rate, and axial length. CONCLUSIONS Even though the POBF values were influenced by gender, BP, and axial length, we could define the normal reference range of POBF in healthy Koreans.