Pulmonary surfactant inhibits cationic liposome-mediated gene delivery to respiratory epithelial cells in vitro.

@article{Duncan1997PulmonarySI,
  title={Pulmonary surfactant inhibits cationic liposome-mediated gene delivery to respiratory epithelial cells in vitro.},
  author={J E Duncan and Jeffrey A. Whitsett and Ann D Horowitz},
  journal={Human gene therapy},
  year={1997},
  volume={8 4},
  pages={431-8}
}
Cationic lipid-mediated transfection of the alveolar epithelium in vivo will require exposure of plasmid DNA and cationic lipids to endogenous surfactant lipids and proteins in the alveolar space. Effects of pulmonary surfactant and of surfactant constituents on transfection in vitro of two respiratory epithelial cell lines (MLE-15 and H441) with a plasmid encoding the luciferase reporter gene were studied using two cationic lipid formulations: 1,2-dimyristyloxypropyl-3-dimethyl-hydroxyethyl… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 17 extracted citations

Similar Papers

Loading similar papers…