Pulmonary diffusing capacities for oxygen-labeled CO2 and nitric oxide in rabbits.

@article{Heller1998PulmonaryDC,
  title={Pulmonary diffusing capacities for oxygen-labeled CO2 and nitric oxide in rabbits.},
  author={Hartmut Heller and Gabriel Fuchs and Klaus-Dieter Schuster},
  journal={Journal of applied physiology},
  year={1998},
  volume={84 2},
  pages={
          606-11
        }
}
We determined the pulmonary diffusing capacity (DL) for 18O-labeled CO2 (C18O2) and nitric oxide (NO) to estimate the membrane component of the respective gas conductances. Six anesthetized paralyzed rabbits were ventilated by a computerized ventilatory servo system. Single-breath maneuvers were automatically performed by inflating the lungs with gas mixtures containing 0.9% C18O2 or 0.05% NO in nitrogen, with breath-holding periods ranging from 0 to 1 s for C18O2 and from 2 to 8 s for NO. The… 
View on PubMed
jap.physiology.org
6 Citations
Background Citations
2

Figures and Tables from this paper

6 Citations

Pulmonary membrane diffusing capacity and capillary blood volume measured during exercise from nitric oxide uptake.

Results support DLNO as a direct measure of pulmonary membrane diffusing capacity, allowing the estimation of Vc in a single rebreathing maneuver during exercise, and can be applied clinically in the investigation of pulmonary microvascular regulation.

Labeled carbon dioxide (C18O2): an indicator gas for phase II in expirograms.

Overall, power moment analysis provides a more comprehensive view on phase II of single-breath expirograms than conventional dead space volume determinations and may be useful for respiratory physiology studies as well as for the study of diseased lungs.

Inducible nitric oxide synthase in the lung and exhaled nitric oxide after hyperoxia.

It is concluded thathyperoxia induces iNOS expression in the lung without an increase in NO concentration in the exhaled air, suggesting that NO is not synthesized in rats exposed to hyperoxia.

Inducible nitric oxide synthase in the lung and exhaled nitric oxide after hyperoxia.

It is concluded thathyperoxia induces iNOS expression in the lung without an increase in NO concentration in the exhaled air, suggesting that NO is not synthesized in rats exposed to hyperoxia.

Pulmonary NO and C18O2 uptake during pressure-induced lung expansion in rabbits.

How to calculate the single-breath nitric oxide diffusing capacity in rabbits

The deviation betweenDL,NO calculated from the conventional methods and the reference decreased linearly with increasing duration of NO uptake (Δt), qualifying the common prediction that the latter method yields DL,NO values of the highest accuracy.

References

SHOWING 1-10 OF 30 REFERENCES

Pulmonary diffusing capacities for nitric oxide and carbon monoxide determined by rebreathing in dogs.

The results suggest that the combined effects of diffusion and chemical reaction with hemoglobin limit alveolar-capillary transport of CO in anesthetized paralyzed supine dogs.

A simultaneous single breath measurement of pulmonary diffusing capacity with nitric oxide and carbon monoxide.

DLNO should be influenced much less by reaction with haemoglobin, and perhaps represents a better index for the diffusing capacity of the alveolar-capillary membrane (Dm) than DLCO.

Determination of lung capillary blood volume and membrane diffusing capacity in man by the measurements of NO and CO transfer.

Kinetics of pulmonary CO2 transfer studied by using labeled carbon dioxide C16O18O.

Diffusion limitation and limitation by chemical reactions during alveolar-capillary transfer of oxygen-labeled CO2.

Evaluation of lung diffusing capacity by physiological and morphometric techniques.

Analysis of the potential errors in the techniques suggests that the correct value for DmCO is substantially higher than that commonly estimated by use of physiological techniques and that the explanation for the difference is due to a number of factors that can influence the binding of CO to hemoglobin under in vivo conditions.

On-line measurement of nitric oxide generation in buffer-perfused rabbit lungs.

In buffer-perfused rabbit lungs, total NO generation may be monitored by continuous analysis of NO exhalation and perfusate NOx accumulation by chemiluminescence detection and immediate increase of lung NO generation in response to A-23187 challenge and inhibition by NG-monomethyl-L-arginine were demonstrated.

Intrapulmonary gas mixing in excised dog lung lobes studied by simultaneous washout of two inert gases.

Pulmonary diffusion limitation after prolonged strenuous exercise.

Total CA activity in isolated perfused guinea pig lung by 18O-exchange method.

The rate of exchange of 18O between alveolar CO2 and lung water was measured in isolated perfused guinea pig lungs to quantify carbonic anhydrase (CA) activity, indicating the CA available to pulmonary capillary plasma is approximately 10% of the total lung CA activity, in agreement with published measurements on the homogenized lung.