Pullulan production by Aureobasidium pullulans cells immobilized on ECTEOLA-cellulose

  title={Pullulan production by Aureobasidium pullulans cells immobilized on ECTEOLA-cellulose},
  author={Thomas P. West},
  journal={Annals of Microbiology},
  • T. West
  • Published 13 August 2010
  • Biology, Engineering
  • Annals of Microbiology
Cells of the fungus Aureobasidium pullulans ATCC 42023 were immobilized by adsorption on the ion-exchange resin ECTEOLA (epichlorohydrin triethanolamine)-cellulose and the immobilized cells were examined for their ability to produce the polysaccharide pullulan using batch fermentation. It was found that the cells immobilized on the ECTEOLA-cellulose at pH 2.0 produced higher pullulan levels than those cells immobilized at pH 3.0, 4.0, 5.0, 6.0 and 7.0 after 72 h at 30°C. The pH 2.0-immobilized… 

Pullulan Production by Aureobasidium pullulans ATCC 201253 Cells Adsorbed onto Cellulose Anion and Cation Exchangers

  • T. West
  • Biology, Engineering
    ISRN microbiology
  • 2012
The anion exchanger phosphocellulose and the cation exchanger triethylaminoethyl cellulose were used to immobilize cells of the fungus Aureobasidium pullulans ATCC 201253 and the adsorbed cells were

Pullulan production by Aureobasidium pullulans cells immobilized in chitosan beads

  • T. West
  • Biology, Engineering
    Folia Microbiologica
  • 2011
The productivity of the immobilized cells increased during the second production cycle while its pullulan content decreased, and the level of cell leakage from the support remained unchanged for both production cycles.

Highly concentrated populations of Aureobasidium pullulans cells in biocatalytic pullulan production processes

Analysis of the obtained data confirms that cell immobilization in a PVA gel for the production of pullulan allows us to shorten the duration of operating cycles in similar processes by a factor of 1.4 while reaching a comparable yield of the target product.

Production of the Polysaccharide Pullulan by Aureobasidium pullulans Cell Immobilization

  • T. West
  • Biology, Engineering
  • 2022
Carrier-binding techniques may be more effective than entrapment techniques for A. pullulans cell immobilization, since carrier-binding is less likely to affect the pullulan content of the polysaccharide being synthesized.

Effect of carbon source on polysaccharide production by alginate‐entrapped Aureobasidium pullulans ATCC 42023 cells

  • T. West
  • Engineering, Biology
    Journal of basic microbiology
  • 2011
The production of the exopolysaccharide pullulan using entrapped cells of the fungus Aureobasidium pullulans ATCC 42023 was investigated relative to carbon source and independent of carbon source, the entrapping fungal cells exhibited a higher yield during the initial cycle than the second production cycle.

Efficient pullulan production by bioconversion using Aureobasidium pullulans as the whole-cell catalyst

Results indicated that pullingulan bioconversion using A. pullulans CCTCC M 2012259 as the whole-cell catalyst is an attractive approach for efficient pullulan production and can be applied for the production of other polysaccharides.

Production and applications of pullulan

Phylogenetic classification of Aureobasidium pullulans strains for production of feruloyl esterase

Production of both xylanase and feruloyl esterase are associated with A. pullulans strains in phylogenetic clade 8, which is thus a promising source of enzymes with potential biotechnological applications.



Use of adsorption in immobilizing fungal cells for pullulan production

It was shown that cells immobilized on the supports at pH 2.0 could be used for two 7 day cycles of pullulan production although polysaccharide levels were found to decrease during the second cycle.

Biosynthesis of pullulan using immobilized Aureobasidium pullulans cells

The immobilization of Aureobasidium pullulans by adsorption on solid supports and entrapment in open pore polyurethane foam showed similar fermentation characteristics and could be repeatedly used for pullulan biosynthesis.

Fungal cell immobilization on ion exchange resins for pullulan production

Pullulan production by the fungal cells immobilization on triethylaminoethyl-cellulose was slightly greater than by the cells immobilized on diethylamino methyl- cellulose or phosphocellulOSE when the corn syrup-grown cells were adsorbed at pH 2.0.

Polysaccharide production by immobilized Aureobasidium pullulans cells in batch bioreactors.

Cells of the fungus Aureobasidium pullulans ATCC 201253 were entrapped within 4% agar cubes or 5% calcium alginate beads and were examined for their production of the polysaccharide pullulan in batch

Polysaccharide production by sponge‐immobilized cells of the fungus Aureobasidium pullulans

It was found that fungal cells grown on corn syrup, sucrose or glucose as a carbon source could be immobilized in sponge cubes and that comparable cell weights and viable cell concentrations were immobilized.

Exopolysaccharide production by entrapped cells of the fungus Aureobasidium pullulans ATCC 201253

  • T. West
  • Biology, Engineering
    Journal of basic microbiology
  • 2000
The production of the exopolysaccharide pullulan using entrapped cells of the fungus Aureobasidium pullulans ATCC 201253 was investigated and fungal cells entrapping in carrageenan demonstrated a lower degree of leakage than did thoseEntrapped in agarose.

Flocculant and chemical properties of a polysaccharide from Pullularia pullulans.

Structural studies on the fractions of purified polysaccharide by methylation and by periodate oxidation techniques prove that PP-floc is linear and composed of alpha-(1 --> 4) and alpha-1 --> 6) glucopyranosyl units in the approximate ratio of 2:1.

Polysaccharide production by a reduced pigmentation mutant of the fungus Aureobasidium pullulans

A reduced pigmentation mutant was isolated from Aureobasidium pullulans ATCC 42023 by chemical mutagenesis and was subsequently characterized and it was noted that the pullulan content of the polysaccharide produced by the parent or mutant strain was lower for sucrose- grown cells than for glucose-grown cells.

Ethanol fermentation by ionically adsorbed Zymomomas mobilis: Environmental effects on cell immobilization

The results suggest that the amount of cells adsorbed during a fermentation process is different from that found from adsorption isotherm data, and application of equilibrium adsorptive data to actual fermentations should be done with caution.

Exopolysaccharide production by free and immobilized microbial cultures