Pullulan Production by Aureobasidium pullulans ATCC 201253 Cells Adsorbed onto Cellulose Anion and Cation Exchangers
@article{West2012PullulanPB, title={Pullulan Production by Aureobasidium pullulans ATCC 201253 Cells Adsorbed onto Cellulose Anion and Cation Exchangers}, author={Thomas P. West}, journal={ISRN Microbiology}, year={2012}, volume={2012} }
The anion exchanger phosphocellulose and the cation exchanger triethylaminoethyl cellulose were used to immobilize cells of the fungus Aureobasidium pullulans ATCC 201253 and the adsorbed cells were subsequently investigated for their ability to produce the polysaccharide pullulan using batch fermentation. The cells adsorbed on the triethylaminoethyl cellulose at pH 7.5 produced higher pullulan levels than those cells immobilized on phosphocellulose at pH 4.0 for 2 cycles of 168 h at 30 °C…
6 Citations
Production of the Polysaccharide Pullulan by Aureobasidium pullulans Cell Immobilization
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Carrier-binding techniques may be more effective than entrapment techniques for A. pullulans cell immobilization, since carrier-binding is less likely to affect the pullulan content of the polysaccharide being synthesized.
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Analysis of the obtained data confirms that cell immobilization in a PVA gel for the production of pullulan allows us to shorten the duration of operating cycles in similar processes by a factor of 1.4 while reaching a comparable yield of the target product.
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