Pullulan Production by Aureobasidium pullulans ATCC 201253 Cells Adsorbed onto Cellulose Anion and Cation Exchangers
@article{West2012PullulanPB,
title={Pullulan Production by Aureobasidium pullulans ATCC 201253 Cells Adsorbed onto Cellulose Anion and Cation Exchangers},
author={Thomas P. West},
journal={ISRN Microbiology},
year={2012},
volume={2012}
}The anion exchanger phosphocellulose and the cation exchanger triethylaminoethyl cellulose were used to immobilize cells of the fungus Aureobasidium pullulans ATCC 201253 and the adsorbed cells were subsequently investigated for their ability to produce the polysaccharide pullulan using batch fermentation. The cells adsorbed on the triethylaminoethyl cellulose at pH 7.5 produced higher pullulan levels than those cells immobilized on phosphocellulose at pH 4.0 for 2 cycles of 168 h at 30 °C…
6 Citations
Production of the Polysaccharide Pullulan by Aureobasidium pullulans Cell Immobilization
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References
SHOWING 1-10 OF 25 REFERENCES
Pullulan production by Aureobasidium pullulans cells immobilized on ECTEOLA-cellulose
- Biology, EngineeringAnnals of Microbiology
- 2010
It was found that the cells immobilized on the ECTEOLA-cellulose at pH 2.0 produced higher pullulan levels than those cells immobilizing at pH 3.0, which was not statistically significant.
Pullulan production by Aureobasidium pullulans cells immobilized in chitosan beads
- Biology, EngineeringFolia Microbiologica
- 2011
The productivity of the immobilized cells increased during the second production cycle while its pullulan content decreased, and the level of cell leakage from the support remained unchanged for both production cycles.
Biosynthesis of pullulan using immobilized Aureobasidium pullulans cells
- Biology, EngineeringBiotechnology and bioengineering
- 1989
The immobilization of Aureobasidium pullulans by adsorption on solid supports and entrapment in open pore polyurethane foam showed similar fermentation characteristics and could be repeatedly used for pullulan biosynthesis.
Fungal cell immobilization on ion exchange resins for pullulan production
- Biology
- 1996
Pullulan production by the fungal cells immobilization on triethylaminoethyl-cellulose was slightly greater than by the cells immobilized on diethylamino methyl- cellulose or phosphocellulOSE when the corn syrup-grown cells were adsorbed at pH 2.0.
Use of adsorption in immobilizing fungal cells for pullulan production
- Biology, Engineering
- 1996
It was shown that cells immobilized on the supports at pH 2.0 could be used for two 7 day cycles of pullulan production although polysaccharide levels were found to decrease during the second cycle.
Polysaccharide production by sponge‐immobilized cells of the fungus Aureobasidium pullulans
- Biology, Engineering
- 1996
It was found that fungal cells grown on corn syrup, sucrose or glucose as a carbon source could be immobilized in sponge cubes and that comparable cell weights and viable cell concentrations were immobilized.
Effect of carbon source on polysaccharide production by alginate‐entrapped Aureobasidium pullulans ATCC 42023 cells
- Engineering, BiologyJournal of basic microbiology
- 2011
The production of the exopolysaccharide pullulan using entrapped cells of the fungus Aureobasidium pullulans ATCC 42023 was investigated relative to carbon source and independent of carbon source, the entrapping fungal cells exhibited a higher yield during the initial cycle than the second production cycle.
Ethanol fermentation by ionically adsorbed Zymomomas mobilis: Environmental effects on cell immobilization
- ChemistryBiotechnology and bioengineering
- 1987
The results suggest that the amount of cells adsorbed during a fermentation process is different from that found from adsorption isotherm data, and application of equilibrium adsorptive data to actual fermentations should be done with caution.
Immobilization of Acetobacter acoti on cellulose ion exchangers: Adsorption isotherms
- BiologyBiotechnology and bioengineering
- 1986
The exceptionally high normalized adsorption capacity of ECTEOLA‐cellulose, 261 mg dry wt/meq, may be explained by an interaction between the cell wall and the polyglyceryl chains of the exchanging groups in addition to the electrostatic effects.
Flocculant and chemical properties of a polysaccharide from Pullularia pullulans.
- ChemistryApplied microbiology
- 1973
Structural studies on the fractions of purified polysaccharide by methylation and by periodate oxidation techniques prove that PP-floc is linear and composed of alpha-(1 --> 4) and alpha-1 --> 6) glucopyranosyl units in the approximate ratio of 2:1.