Pulling on single molecules

  title={Pulling on single molecules},
  author={Natalie de Souza},
  journal={Nature Methods},
Single-molecule methods to apply and measure force and displacement are expanding mechanobiology. 
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Reliable extraction of energy landscape properties from critical force distributions
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Monachino et al. review recent developments in single-molecule biophysical approaches and the cell biological advances they allow.
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Pushing, pulling, and squeezing our way to understanding mechanotransduction.
This work highlights and reviews experimental methods for investigating mechanotransduction across multiple length scales and discusses approaches for determining how molecular-scale mechanical stimuli arise in vivo in the context of complex, multicellular tissue structures. Expand
Acoustic tweezers for studying intracellular calcium signaling in SKBR-3 human breast cancer cells.
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Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics
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Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy
These techniques are described and illustrated with examples highlighting current capabilities and limitations of single-molecule force spectroscopy. Expand