Pulling on single molecules

@article{Souza2012PullingOS,
  title={Pulling on single molecules},
  author={Natalie de Souza},
  journal={Nature Methods},
  year={2012},
  volume={9},
  pages={873-877}
}
Single-molecule methods to apply and measure force and displacement are expanding mechanobiology. 
Highly-parallel microfluidics-based force spectroscopy on single cytoskeletal motors
TLDR
A highly-parallel, microfluidics-based method that allows for rapid collection of force-dependent motility parameters of cytoskeletal motors with two orders of magnitude improvement in throughput compared to currently available methods is introduced. Expand
Imaging-assisted hydrogel formation for single cell isolation
TLDR
The method presented here offers a mask-free, cost-efficient and easy-to-use alternative to many currently existing surface-based cell-sorting techniques, and has the potential to impact the field of cell culturing and isolation, e.g. single cell genomics and proteomics. Expand
Polymer mechanochemistry-enabled pericyclic reactions
Over the past decades, it became clear that next to heat and light, pericyclic reactions can be induced mechanochemically when the reacting motifs are embedded as latent force-responsive groupsExpand
Viologen Tweezers to Probe the Force of Individual Donor-Acceptor π-Interactions.
TLDR
A molecular tweezer is designed and synthesized that is able to encapsulate π-donors and also demonstrated a precise opening extension, revealing the opening of individual viologen tweezers with an unambiguous extension. Expand
Live cell measurements of interaction forces and binding kinetics between Discoidin domain receptor 1 and collagen I with atomic force microscopy.
TLDR
A biophysical method to isolate and measure specific interactions between DDR1 and collagen in live cells at the single molecule level using atomic force microscopy shows promise in quantifying kinetics and receptor density on live cells, as well as evaluating potential therapeutic agents. Expand
Reliable extraction of energy landscape properties from critical force distributions
The structural dynamics of a biopolymer is governed by a process of diffusion through its conformational energy landscape. In pulling experiments using optical tweezers, features of the energyExpand
Watching cellular machinery in action, one molecule at a time
Monachino et al. review recent developments in single-molecule biophysical approaches and the cell biological advances they allow.
Microfabricated tissues for investigating traction forces involved in cell migration and tissue morphogenesis
TLDR
The fundamentals of traction forces, their quantification, and the use of microfabricated tissues designed to study these forces during cell migration and tissue morphogenesis are reviewed. Expand
Pushing, pulling, and squeezing our way to understanding mechanotransduction.
TLDR
This work highlights and reviews experimental methods for investigating mechanotransduction across multiple length scales and discusses approaches for determining how molecular-scale mechanical stimuli arise in vivo in the context of complex, multicellular tissue structures. Expand
Acoustic tweezers for studying intracellular calcium signaling in SKBR-3 human breast cancer cells.
TLDR
It is found that intracellular calcium elevations in SKBR-3 cells are elicited by attaching an acoustically trapped FNT-coated microbead and are suppressed by either extracellular calcium elimination or phospholipase C (PLC) inhibition. Expand
...
1
2
...

References

SHOWING 1-3 OF 3 REFERENCES
Five challenges to bringing single-molecule force spectroscopy into living cells
TLDR
The problems and challenges that need to be addressed to bring single-molecule force spectroscopy technologies into living cells and to learn how cellular machinery is controlled in vivo are discussed. Expand
Measuring mechanical tension across vinculin reveals regulation of focal adhesion dynamics
TLDR
Developing a calibrated biosensor that measures forces across specific proteins in cells with piconewton (pN) sensitivity reveals that FA stabilization under force requires both vinculin recruitment and force transmission, and that, surprisingly, these processes can be controlled independently. Expand
Single-molecule force spectroscopy: optical tweezers, magnetic tweezers and atomic force microscopy
TLDR
These techniques are described and illustrated with examples highlighting current capabilities and limitations of single-molecule force spectroscopy. Expand