Pu . rification and Properties of a Nucleosicle Phosphotransferase from Carrot *

  • A. U.S., ELINOR F. BHUNSGRABERAKD ERWIN CHARGAFF
  • Published 2003
A simple procedure affording a 940-fold purification of the nucleoside phosphotransferase from carrot is described. Preliminary evidence indicates that, by an additional fractionation through a Sephadex column, a further 5to 6-fold increase in activity can be effected. The particle weight of the enzyme varies with pH. At the pH optimum of 5.0 one species of… CONTINUE READING