Ptychography – a label free, high-contrast imaging technique for live cells using quantitative phase information

@inproceedings{Marrison2013PtychographyA,
  title={Ptychography – a label free, high-contrast imaging technique for live cells using quantitative phase information},
  author={Joanne Marrison and Lotta R{\"a}ty and Poppy E Marriott and Peter J O'Toole},
  booktitle={Scientific reports},
  year={2013}
}
Cell imaging often relies on synthetic or genetic fluorescent labels, to provide contrast which can be far from ideal for imaging cells in their in vivo state. We report on the biological application of a, label-free, high contrast microscopy technique known as ptychography, in which the image producing step is transferred from the microscope lens to a high-speed phase retrieval algorithm. We demonstrate that this technology is appropriate for label-free imaging of adherent cells and is… CONTINUE READING
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