PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A

@article{Ktistakis1991PtK1CC,
  title={PtK1 cells contain a nondiffusible, dominant factor that makes the Golgi apparatus resistant to brefeldin A},
  author={Nicholas T Ktistakis and Michael G. Roth and George S Bloom},
  journal={The Journal of Cell Biology},
  year={1991},
  volume={113},
  pages={1009 - 1023}
}
Brefeldin A (BFA) was shown in earlier studies of numerous cell types to inhibit secretion, induce enzymes of the Golgi stacks to redistribute into the ER, and to cause the Golgi cisternae to disappear. Here, we demonstrate that the PtK1 line of rat kangaroo kidney cells is resistant to BFA. The drug did not disrupt the morphology of the Golgi complex in PtK1 cells, as judged by immunofluorescence using antibodies to 58- (58K) and 110-kD (beta-COP) Golgi proteins, and by fluorescence microscopy… CONTINUE READING

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