PslD is a secreted protein required for biofilm formation by Pseudomonas aeruginosa.

  title={PslD is a secreted protein required for biofilm formation by Pseudomonas aeruginosa.},
  author={Andrea Campisano and Christine Schroeder and Mirle Schemionek and Joerg Overhage and Bernd H A Rehm},
  journal={Applied and environmental microbiology},
  volume={72 4},
The function of pslD, which is part of the psl operon from Pseudomonas aeruginosa, was investigated in this study. The psl operon is involved in exopolysaccharide biosynthesis and biofilm formation. An isogenic marker-free pslD deletion mutant of P. aeruginosa PAO1 which was deficient in the formation of differentiated biofilms was generated. Expression of only the pslD gene coding region restored the wild-type phenotype. A C-terminal, hexahistidine tag fusion enabled the identification of PslD… CONTINUE READING


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