Pseudotargeted metabolomics method and its application in serum biomarker discovery for hepatocellular carcinoma based on ultra high-performance liquid chromatography/triple quadrupole mass spectrometry.

@article{Chen2013PseudotargetedMM,
  title={Pseudotargeted metabolomics method and its application in serum biomarker discovery for hepatocellular carcinoma based on ultra high-performance liquid chromatography/triple quadrupole mass spectrometry.},
  author={Shili Chen and Hongwei Kong and Xin Lu and Yong An Li and Peiyuan Yin and Zhongda Zeng and Guowang Xu},
  journal={Analytical chemistry},
  year={2013},
  volume={85 17},
  pages={8326-33}
}
Untargeted analysis performed using full-scan mass spectrometry (MS) coupled with liquid chromatography (LC) is commonly used in metabolomics. Although they are commonly employed, full-scan MS methods such as quadrupole-time-of-flight (Q-TOF) MS have been restricted by various factors including their limited linear range and complicated data processing. LC coupled with triple quadrupole (QQQ) MS operated in the multiple reaction monitoring (MRM) mode is the gold standard for metabolite… CONTINUE READING