Pseudophosphorylation of the smooth muscle 20 000 dalton myosin light chain. An artifact due to protein modification.

Abstract

The use of isoelectric focusing as a technique for quantifying the stoichiometry of phosphorylation of the 20 kDa smooth muscle myosin light chain (LC20) was found to overestimate true levels of phosphorylation under certain conditions due to the occurrence of LC20 charge modification. Modification of unphosphorylated LC20 produced a band of… (More)

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