Pseudomonas predators: understanding and exploiting phage–host interactions

@article{Smet2017PseudomonasPU,
  title={Pseudomonas predators: understanding and exploiting phage–host interactions},
  author={Jeroen De Smet and Hanne Hendrix and Bob G. Blasdel and Katarzyna Danis-Wlodarczyk and Rob Lavigne},
  journal={Nature Reviews Microbiology},
  year={2017},
  volume={15},
  pages={517-530}
}
Species in the genus Pseudomonas thrive in a diverse set of ecological niches and include crucial pathogens, such as the human pathogen Pseudomonas aeruginosa and the plant pathogen Pseudomonas syringae. The bacteriophages that infect Pseudomonas spp. mirror the widespread and diverse nature of their hosts. Therefore, Pseudomonas spp. and their phages are an ideal system to study the molecular mechanisms that govern virus–host interactions. Furthermore, phages are principal catalysts of host… 
Bacterial Diversification in the Light of the Interactions with Phages: The Genetic Symbionts and Their Role in Ecological Speciation
TLDR
This framework builds upon classical morphological and molecular taxonomy by also considering bacteria and their phages as a unit of evolutionary selection and will advance the understanding of bacterial evolution.
Phage Morons Play an Important Role in Pseudomonas aeruginosa Phenotypes
TLDR
It is found that many morons confer selective advantages for the bacterial host, some of which correlate with increased bacterial virulence.
Emerging Phage Resistance in Pseudomonas aeruginosa PAO1 Is Accompanied by an Enhanced Heterogeneity and Reduced Virulence
TLDR
These findings support phage application in therapy, although the overall statement that phage treatment selects the less virulent bacterial population should be further verified using a bigger collection of clinical strains.
Impact of phages on soil bacterial communities and nitrogen availability under different assembly scenarios
TLDR
The results show that changes in phage pressure have the potential to impact soil bacterial community composition and diversity, and a positive effect of active phages on the soil ammonium concentration in a few treatments indicates that increased phages pressure may also be important for soil functions.
Phage efficacy in infecting dual-strain biofilms of Pseudomonas aeruginosa
TLDR
It is revealed that living in a spatially structured population can protect bacteria against phage infection, while the presence of competing strains may instead reduce the likelihood of evolving resistance to phage, if encountered.
Spatial structure affects phage efficacy in infecting dual-strain biofilms of Pseudomonas aeruginosa
TLDR
It is reported that living in a spatially structured population can protect bacteria against phage infection, which is mainly due to slower growth at colony centre rather than any additional protection provided by presence of insensitive strain.
Bacterial biodiversity drives the evolution of CRISPR-based phage resistance in Pseudomonas aeruginosa
TLDR
It is shown that coexistence with other human pathogens amplifies the fitness trade-offs associated with phage receptor mutation, and therefore tips the balance in favour of CRISPR-based resistance evolution, with key implications for bacterial fitness and virulence.
The Age of Phage: Friend or Foe in the New Dawn of Therapeutic and Biocontrol Applications?
TLDR
This review aims to provide a balanced perspective on bacteriophages by outlining advantages, challenges, and future steps needed in order to boost their therapeutic and biocontrol potential, while also providing insight on their potential role in contributing to bacterial evolution and survival.
Deconstructing the Phage–Bacterial Biofilm Interaction as a Basis to Establish New Antibiofilm Strategies
TLDR
This work highlights how natural systems have previously inspired new antibiofilm design strategies, e.g., by utilizing exopolysaccharide degrading enzymes and peptidoglycan hydrolases, and proposes new potential approaches including phage-encoded DNases to target extracellular DNA, as well asphage-mediated inhibitors of cellular communication.
Bacterial biodiversity drives the evolution of CRISPR-based phage resistance
TLDR
It is shown that coexistence with other human pathogens amplifies the fitness trade-offs associated with the mutation of phage receptors, and therefore tips the balance in favour of the evolution of CRISPR-based resistance, with key implications for bacterial fitness and virulence.
...
...

References

SHOWING 1-10 OF 199 REFERENCES
Temperate phages both mediate and drive adaptive evolution in pathogen biofilms
TLDR
It is shown that temperate phages found in human infections accelerated pathogen evolution by increasing the supply of beneficial mutations and imposing strong selection on bacterial populations, which accelerated the loss of clinically important virulence-related bacterial traits, including motility and quorum sensing.
A new perspective on lysogeny: prophages as active regulatory switches of bacteria
TLDR
Another type of bacterium–phage interaction is discussed, active lysogeny, in which phages or phage-like elements are integrated into the bacterial chromosome within critical genes or operons and serve as switches that regulate bacterial genes via genome excision.
Evolution of Pseudomonas aeruginosa Virulence as a Result of Phage Predation
TLDR
The results of this study indicate a significant change in the in vitro virulence of P. aeruginosa following phage predation and highlight the need for caution in the selection and design of phages and phage cocktails for therapeutic use.
Importance of prophages to evolution and virulence of bacterial pathogens
TLDR
The purpose of this review is to give an overview of the various ways by which prophages change the lifestyle and boost virulence of some of the most dangerous bacterial pathogens.
Phages can constrain protist predation-driven attenuation of Pseudomonas aeruginosa virulence in multienemy communities
TLDR
It is suggested that protist selection can be a strong coincidental driver of attenuated bacterial virulence, and that phages can constrain this effect owing to effects on population dynamics and conflicting selection pressures.
Revenge of the phages: defeating bacterial defences
TLDR
The various tactics that are used by phages to overcome bacterial resistance mechanisms, including adsorption inhibition, restriction–modification, CRISPR–Cas (clustered regularly interspaced short palindromic repeats–CRISPR-associated proteins) systems and abortive infection are described.
Pseudomonas genomes: diverse and adaptable.
Members of the genus Pseudomonas inhabit a wide variety of environments, which is reflected in their versatile metabolic capacity and broad potential for adaptation to fluctuating environmental
The potential role of endogenous bacteriophages in controlling invading pathogens
TLDR
It is hypothesized that phages could act in concert with the immune system in immunosurveillance against bacteria, viruses and cancer.
The role of interactions between phage and bacterial proteins within the infected cell: a diverse and puzzling interactome.
TLDR
New research into the antibacterial potential of bacteriophage-host interactions indicates that phage early proteins seem to target a wide variety of processes in the host cell - many of them non-essential.
Prophages mediate defense against phage infection through diverse mechanisms
TLDR
This study represents the first systematic approach to address how a population of prophages influences phage resistance and bacterial behavior in P. aeruginosa through a surface modification that is cost-free for the bacterial host in a natural soil environment and in a Caenorhabditis.
...
...